A stopped-flow/flow-injection system for automation ofα2macroglobulin kinetic studies

A stopped-flow/flow-injection system for automation of α2 macroglobulin kinetic studies

Automation of a flow-injection system for multispeciation

Introduction Differentiating between the two most frequent oxidation states of chromium is fundamental in speciation studies of this element on account of the toxicity of hexavalent chromium. In fact, most of the works on this element published so far deal solely with this aspect [1]. Flow-injection analysis (FIA) has been used several times for determining this element in one [2 and 3] or in i...

Some problems concerning mixers and detectors for stopped flow kinetic studies.

This conference is taking place on the 55th anniversary of the introduction of rapid mixing methods to the field of biology by Hartridge and Roughton (1923a). Their specific aim was to study the reactions of hemoglobin with its ligands (Hartridge and Roughton, 1923b). The tenacity with which nature holds onto its secrets is nowhere better exemplified than with this remarkable molecule. The very...

Data acquisition system for chemical kinetic studies

A microcomputer-interfaced data acquisition system for chemical kinetics (interfacing with laboratory analogue instruments) has been developed. Analogue signals from instruments used in kinetics experiments are amplifed by a wide-range adjustable high-gain operational amplifier and smoothed by an op-based filter, and then digitized at rates of up to 10(4) samples per channel by an ADC 0816 digi...

A dedicated automatic dilutor for the automation of α2 macroglobulin kinetic studies

In 1979, evidence was produced by Topping and associates [1, 2 and 3] that any one of at least seven different types of 2 macroglobulin (2M) is to be found circulating in human blood. Serum samples from healthy individuals showed a reproducible distribution amongst the seven categories, which differed markedly from the distribution obtained from a group of patients suffering from a variety of p...